Journal: British Journal of Cancer

Article Title: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?

doi: 10.1038/sj.bjc.6600597

Figure Lengend Snippet: Flow cytometric examination of the induction of CD103 expression by T cells in MLR culture. ( A ) Shows the time course for induction of CD103 on lymphocytes cultured in MLR with TGFβ 1 added on day 3 (solid line) or without TGFβ 1 (dotted line). The data points show the mean of 10-fold determinations; the error bars show the s.e.m. ( B ) Shows results from a representative two-parameter flow cytometric dot-plot for viable cells harvested from a day 9 MLR which was supplemented with TGFβ 1 on day 3. It can be seen that the majority (75.6%) of the CD8 T cells at this time point also express CD103.

Article Snippet: Studies were performed using murine anti-human E-cadherin (Clone 67A4; Immunotech), murine anti-human ICAM-1 (Clone84H10; Serotec), murine anti-human CD45 : RPE (Clone T29/33; DAKO), murine anti-human CD103 (Clone Ber-ACT8; DAKO), murine anti-human CD3 (OKT3; Janssen–Cilag, Buckinghamshire, UK), murine anti-human CD11a (α subunit of LFA-1, Clone MHM24; DAKO), murine anti-human CD18 (β subunit of LFA-1, Clone MHM23; DAKO), murine IgG 1 (isotype control X0931, DAKO).

Techniques: Expressing, Cell Culture

Journal: British Journal of Cancer

Article Title: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?

doi: 10.1038/sj.bjc.6600597

Figure Lengend Snippet: Examination of the adhesion of activated T lymphocytes to Panc-1 cells. ( A ) Demonstration of binding of T cells from non-TGFβ 1 supplemented (open bars) and TGFβ 1 -supplemented (solid bars) MLR cultures to wild-type and E-cadherin transfected Panc-1 cells. The bars show the mean of 10-fold determinations; the error bars show the s.e.m. ( B ) Examination of antibody-blockade of the adhesion of T cells from a TGFβ 1 supplemented MLR to wild-type and E-cadherin transfected Panc-1 cells. Control assays are indicated by open bars, solid bars show blockade of CD103 and hatched bars show blockade of ICAM-1. The bars show the mean of 10-fold determinations; the error bars show the s.e.m.

Article Snippet: Studies were performed using murine anti-human E-cadherin (Clone 67A4; Immunotech), murine anti-human ICAM-1 (Clone84H10; Serotec), murine anti-human CD45 : RPE (Clone T29/33; DAKO), murine anti-human CD103 (Clone Ber-ACT8; DAKO), murine anti-human CD3 (OKT3; Janssen–Cilag, Buckinghamshire, UK), murine anti-human CD11a (α subunit of LFA-1, Clone MHM24; DAKO), murine anti-human CD18 (β subunit of LFA-1, Clone MHM23; DAKO), murine IgG 1 (isotype control X0931, DAKO).

Techniques: Binding Assay, Transfection

Journal: British Journal of Cancer

Article Title: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?

doi: 10.1038/sj.bjc.6600597

Figure Lengend Snippet: Investigation of the effect on Panc-1 cytolysis of antibody blockade of ICAM-1 or CD103. The effector T cells were harvested from an MLR performed in the presence of TGFβ 1 and were additionally stimulated with anti-CD3 antibody prior to addition to the target cells. The hatched bar shows control cytolysis of the antigen-presenting cells used for T cell activation, the open bar shows the lysis of E-cadherin transfected Panc-1 in the presence of a control antibody, the shaded bar shows the effect of ICAM-1 blockade, the solid bar shown the effect of CD103 blockade and the horizontally filled bar shows lysis of wild-type Panc-1 cells. The bars show the mean of 10-fold determinations; the error bars show the s.e.m.

Article Snippet: Studies were performed using murine anti-human E-cadherin (Clone 67A4; Immunotech), murine anti-human ICAM-1 (Clone84H10; Serotec), murine anti-human CD45 : RPE (Clone T29/33; DAKO), murine anti-human CD103 (Clone Ber-ACT8; DAKO), murine anti-human CD3 (OKT3; Janssen–Cilag, Buckinghamshire, UK), murine anti-human CD11a (α subunit of LFA-1, Clone MHM24; DAKO), murine anti-human CD18 (β subunit of LFA-1, Clone MHM23; DAKO), murine IgG 1 (isotype control X0931, DAKO).

Techniques: Activation Assay, Lysis, Transfection

Journal: Cell Reports Medicine

Article Title: B cell depletion attenuates CD27 signaling of T helper cells in multiple sclerosis

doi: 10.1016/j.xcrm.2023.101351

Figure Lengend Snippet:

Article Snippet: Anti-human CD103 (clone Ber-ACT8) (151 Eu) , Standard BioTools Inc , Cat# 3151011B; RRID: AB_2756418.

Techniques: Purification, Recombinant, Electron Microscopy, Blocking Assay, Polymer, Mass Cytometry, RNA Sequencing, Software, Imaging, Microscopy

Journal: Frontiers in Immunology

Article Title: Single-cell profiling of T cells uncovers a tissue-resident memory-like T-cell subset associated with bidirectional prognosis for B-cell acute lymphoblastic leukemia

doi: 10.3389/fimmu.2022.957436

Figure Lengend Snippet: Identification of CD103+ T (Trm-like) cells in the peripheral blood of B-ALL patients and healthy individuals through scRNA-seq. (A) . UMAP (uniform manifold approximation and projection) visualization of T-cell single cell clusters from B-ALL patients (left) and healthy individuals (HI; right). Different clusters are depicted with distinct colors. (B) . Dot plot of marker genes for each cell cluster. Color scale indicates the mean of normalized expression of marker genes in each cell type, and dot size is proportional to the percentage of cells within each cell cluster expressing the marker genes. The red box highlights the marker genes for Trm-like cells. (C) . Variation of the proportion of each defined cell type between sample groups (B-ALL vs . HI). (D) . Proportion of Trm-like cells in each assayed sample. Red and green dots represent B-ALL patients and healthy individuals, respectively. Trm-like, tissue-resident memory-like T cell; B-ALL, B cell-acute lymphoblastic leukemia; Teff, effector T cell; Tem, effector memory T cell; Tcm, central memory T cell; Th, T-helper cell; Treg, regulatory T cell; MAIT, mucosal-associated invariant T cell; Tex, exhausted T cell.

Article Snippet: Cell surface and intracellular staining was performed using the following fluorophore-conjugated antibodies: CD45-BUV395 (clone HI30, BD), CD3-AF700 (clone UCHT1, BD), CD4-APC-H7 (clone RPAT4, BD), CD8-Percp-cy5.5 (clone SK1, BioLegend), Ki-67-PE-CF594 (clone Ki-67, BioLegend), PD-1-BV421 (clone MIH4, BD), and CD103-BB515 (clone Ber-ACT8, BD).

Techniques: Marker, Expressing